M. Tech Bio Technology 2nd Semester Syllabus

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MOLECULAR MODELING AND SIMULATION
Subject Code : 14BBT253

IA Marks : 50
No. of Lecture Hrs./ Week : 04 Exam Hrs : 03
Total No. of Lecture Hrs. : 50 Exam Marks : 100

COURSE OBJECTIVES:
To enhance conventional process and to emphasis on system simulation and to correlate with real processes To experience the unexplored area at micro to macro level.

MODULE 1: 10 Hours
Biomolecular Structure and Modeling: Historical Perspective, Introduction to Molecular Modeling, Roots of Molecular modeling in Molecular mechanics. Introduction to X-Ray crystallography and NMR spectroscopy. Introduction to PDB and 3D Structure data, Structure of PDB and other 3D Structure record.
Protein Structure Hierarchy: Structure Hierarchy. Helices – Classic α-Helix and π Helices, Left-Handed α-Helix and Collagen Helix. β- Sheets – Turns and Loops. Supersecondary and Tertiary structure. Complex 3D Networks. Classes in Protein Architecture – Folds, α-Class, Bundles, Folded leaves, Hairpin arrays. β-Class folds, Anti-parallel β domains, parallel and Anti-parallel Combinations. α/β and α+β- Class, α/β Barrels, Open twisted α/β folds, Leucine-rich α/β folds. α+β folds. Quaternary structure. Discussions with case studies.

MODULE 2: 10 Hours
Force Fields: Formulation of the Model and Energy, Quantifying Characteristic Motions, Complex Biomolecular Spectra, Spectra as force constant sources, In-Plane and Out-of-Plane Bending. Bond Length Potentials – Harmonic term, Morse term, Cubic and Quadratic terms.
Bond Angle Potentials – Harmonic and Trigonometric terms, Cross bond stretch / Angle bend terms. Torsional potentials – Origin of rotational barriers, Fourier terms, Torsional parameter Assignment, Improper torsion, Cross dihedral/Bond angle, Dihedral terms. Van der Waals potentials. Rapidly decaying potential. Parameter fitting from experiment. Two parameter calculation protocols. Coulomb potential – Coulomb’s Law. Slowly decaying potential, Dielectric function and Partial charges. Discussions with case studies.

MODULE 3: 10 Hours
Molecular modeling: Modeling basics. Generation of 3D Coordinates Crystal data, Fragment libraries, and conversion of 2D Structural data into 3D form. Force fields, and Geometry optimization. Energy minimizing procedures – Use of Charges, Solvent effects and Quantum Mechanical methods. Computational tools for Molecular modeling. Methods of Conformational analysis – Systematic search procedures, Monte Carlo and molecular dynamics methods. Determining features of proteins – Interaction potential, Molecular electrostatic potential, molecular interaction fields, Properties on molecular surface and Pharmacophore identification. 3D QSAR Methods. Comparative protein
modeling – Conformational properties of protein structure, Types of secondary structural elements, Homologous proteins. Procedures for sequence Alignments, Determination and generation of structurally conserved regions, Construction of structurally variable regions, Side- Chain modeling, Secondary structure prediction, Threading methods. Optimization and Validation of Protein Models with suitable case studies. Computation of the Free Energy: Free energy calculations in Biological Systems – Drug design, Signal transduction, Peptide folding, Membrane protein association, Numerical methods for calculating the potential of mean force, Replica-Exchange-Based Free-
Energy Methods.

MODULE 4: 10 Hours
Membrane Protein Simulations: Membrane proteins and their importance, Membrane protein environments in vivo and in vitro. Modeling a complex environment – Simulation methods for membranes, Membrane protein systems, Complex solvents, Detergent micelles, Lipid bilayers, Self-Assembly and Complex systems. Modeling and Simulation of Allosteric regulation in enzymes – Discussions with case studies.
Electrostatics and Enhanced Solvation Models: Implicit solvent electrostatics in Biomolecular Simulation, New distributed multipole methods. Quantum mechanical principles and applications to force field development with case studies.

MODULE 5: 10 Hours
Virtual Screening and Docking: Introduction to Docking – Preparation of Partners, Compound Library, representation of Proteins and Ligands. Docking Algorithms. Simulation approaches for Protein model. Simulation of a Fluid Phase Lipid Bilayer Membrane, de novo Simulations of the Folding of GCN4 and its Mutants. Building a model of HIV-I Reverse Transcriptase (possible mechanisms for AZT resistance).

COURSE OUTCOMES:
Student will be able to analyse and comprehend the structure and other components of real processes. Also will be able to create the model for various components of life processes.

TEXT BOOKS:
1. Hans-Dieter Höltje, Wolfgang Sippl, Didier Rognan, Gerd Folkers, Molecular Modeling, 2008.
2. Alberte Pullman, Joshua Jortner, Modeling of Bimolecular Structures and Mechanisms, 1995.

REFERENCE BOOKS:
3. Jill P. Mesirov, Klaus Schulten, De Witt L. Sumners, Mathematical Approaches to Biomolecular Structure and Dynamics, 1996.
4. Peter T. Cummings, Phillip R. Westmorland, Brice Carnahan, Foundations of Molecular Modeling and Simulation, Published by American Institute of Chemical Engineers, 2001.
5. Timothy J. Barth, Michael Griebel, David E.Keyes, Risto M. Nieminen, Dirk Roose, Tamar Schlick, New Algorithms for Macromolecular Simulation, Published by Springer, 2006.

MICROBIAL BIOTECHNOLOGY
Subject Code : 14BBT22

IA Marks : 50
No. of Lecture Hrs./ Week : 04 Exam Hrs : 03
Total No. of Lecture Hrs. : 50 Exam Marks : 100

COURSE OBJECTIVES:
Apply and understand the techniques of genetic engineering in the field of microbial biotechnology
Develop methodology for production of various important metabolites along with production of vitamins and other adjuvants
Describe the role of microbe in mineral recovery and fuel production

MODULE 1: 10 Hours
Industrially important microorganisms, aerobic and anaerobic microbial processes, introduction to microbial process development, scale – up and scale-down of microbial processes, Microbial Biotechnology: scope, techniques and examples. World of omics.
Microbial cultures: Isolation of cultures, screening for activities, culture preservation and inoculum development, small-scale liquid and solid state fermentations, strain improvement: the random and empirical approach.

MODULE 2: 10 Hours
Microbes and genetic engineering – Introduction to genetic engineering, producing genetically engineered microorganisms – Escherichia coli and Saccharomyces cerevisiae. Introduction of DNA into bacteria, use of vectors, detection of clone containing desired fragment, expression of clones genes, Recovery and purification of expressed proteins. Production of proteins in yeast, yeast cloning vectors, enhancing the expression of foreign genes in yeast, expression of foreign gene products in secreted form.

MODULE 3: 10 Hours
Aerobic and anaerobic fermentation, types of fermenter, process of fermentation, Microbial culture system – batch culture, fed batch culture, continuous culture, measurement and control of bioprocess parameters, scale-up. Liquid and solid-state fermentation, Downstream processing – Solids removal, primary separation, purification and isolation of products (emphasis to proteins).

MODULE 4: 10 Hours
Production of primary and secondary metabolites, Solvents – Alcohol, Glycerol. Organic acids – Citric acid, acetic acid, L-ascorbic acid, lactic acid. Antibiotics – Penicillins (Penicillin), Cephalosporins (cephalosporin), Aminoglycosides (streptomycin), Tetracyclines (chlortetracycline), Macrolides (erythromycin A). Amino acids – L-glutamic acid, L-lysine, L-tryptophan. Vitamins – Vitamin B12, Riboflavin, Beta-carotene. Beverages from microbes, Microbial polysaccharides and polymers.

MODULE 5: 10 Hours
Microbes in Energy & Environment: Alcohol and methane from biomass, photo-biological hydrogen production, Electricity from biofuel cells. Microbial mining and metal biotechnology – Bioleaching and biosorption.

COURSE OUTCOMES:
After completion of the course, students are able to modify microbes for the production of various commercially important compounds and provide the strategy for production of primary and secondary metabolites.

TEXT BOOKS:
1. Alexander N glazer, Hiroshi nikaido, Microbial Biotechnology. 2nd Edition, Cambridge, 2008.
2. L.E. Casida Jr, Industrial microbiology. New age International publisher, 2008.
3. Lee yuan Kun (edi), Microbial biotechnology – Principles and applications World Scientific publisher, 2004.

REFERENCE BOOKS:
4. E I Mansi and Bryce. Fermentation Microbiology and Biotechnology Taylor & Francis, 2004.
5. Michael Waites, Neil Morgan John Rockey and Gary Higton. Industrial Microbiology: An Introduction. Blackwell publishing.

BIOPHARMACEUTICALS
Subject Code : 14BBT23

IA Marks : 50
No. of Lecture Hrs./ Week : 04 Exam Hrs : 03
Total No. of Lecture Hrs. : 50 Exam Marks : 100

COURSE OBJECTIVES:
Illustrate the process and mechanism of biopharmaceutical products. Enhance the knowledge and understanding of emerging aspects of biopharmaceuticals.

MODULE 1: 10 Hours
Introduction to pharmaceutical industry, pharmaceutical products, Biopharmaceuticals & Biotechnology, Biopharmaceuticals: Current status & future prospects. Traditional pharmaceuticals of biological origin. Pharmaceuticals from plant, animal and microbial origin. Drug discovery & development process. Impact of genomics, Patenting, Delivery of biopharmaceuticals, pre-clinical trials, clinical trials, the role of regulatory authorities.

MODULE 2: 10 Hours
Pharmacopeias, good manufacturing practices, manufacturing facilities, clean rooms, water systems, CDS practices, sources of biopharmaceuticals, production of final product & formulation, analysis of final product, labeling and packing, physico-chemical and biological tests applied to biopharmaceuticals, qualitative and quantitative tests for proteins, electrophoresis, HPLC, Mass spectrometry, Immunological approaches for the detection of impurities. Endotoxin testing, BET testing.

MODULE 3: 10 Hours
The cytokines, growth factors, haemopoietic growth factors, growth factors, hormones of therapeutic interest, blood products & therapeutic enzymes (with appropriate case studies). Manufacturing activities for Monoclonal Antibodies, vaccines and adjuvants. Nucleic acid therapeutics, gene therapy, anti-sense technology.

MODULE 4: 10 Hours
Chemical conversion processes, physic-chemical principles in drug metabolism, Action of drugs on CNS, CVS, Recombinant protein and drug development, Nutraceuticals and biological.

MODULE 5: 10 Hours
Product processing and packaging, Scale-up techniques, packaging material science. Validation, stability testing, Quality Control & Quality Assurance, Drug regulatory Affairs. The Food and Drug Administration, European regulations, Drug regulation in Japan, World harmonization of drug approvals.

COURSE OUTCOMES:
After completion of the course, students are able to conceptualize the role of biopharmaceuticals. Exercise better professionalism by incorporating manufacturing of pharmaceutical products.

TEXT BOOKS:
1. Gary Walsh (Second Edition), Biopharmaceuticals: Biochemistry and Biotechnology Wiley Publishers, 2003.
2. Gray Walsh & B. Murphy, Biopharmaceuticals and industrial prospective, Kluwer publishers, 1999.
3. Bernard Glick, Jack Pasternak, Cheryl Patten. Molecular Biotechnology, ASM Press, 2009.

REFERENCE BOOKS:
4. Dann, J.A, Crommelin & Robert D., Sindelar, Pharmaceutical Biotechnology, Taylor & Francis, 2002.
5. Arnold Demain and Julian Davies, Manual of Industrial Microbiology and Biotechnology. ASM Press, 2004.

ADVANCED BIOINFORMATICS
Subject Code : 14BBT24

IA Marks : 50
No. of Lecture Hrs./ Week : 04 Exam Hrs : 03
Total No. of Lecture Hrs. : 50 Exam Marks : 100

COURSE OBJECTIVES:
To have knowledge of molecular and genetic engineering principle to design and predict the structure of novel compounds.
To know the principle of drug design and its applications in proteomics and genomics.
Explore logical and critical thinking ability to solve biological problems with the help of BioPerl and other bioinformatic techniques

MODULE 1: 10 Hours
Basic Concepts of Molecular Biology: Proteins, Nucleic Acids: DNA, RNA. Molecular Genetics: Genes and Genetic code, Transcription, Translation and Protein synthesis, Chromosomes Genome: Maps and Sequences, Sequencing Techniques, Human Genome project, Sequence Databases.

MODULE 2: 10 Hours
Biological Databases: Types of databases – Primary and Secondary biological databases. Primary databases, secondary databases, genotype databases, molecular structure databases and genome databases. Hidden Markov Models: Forward and Backward algorithm, Viterbi algorithm, Applications: Modelling Protein sequence families, multiple alignments.

MODULE 3: 10 Hours
Protein Modeling and Insilico Drug Design: Protein structure, signal peptides, transmembrane proteins, analysis of protein structures. Protein modeling, modeling protein structures using High Throughput methods. Insilico drug design, Virtual Library design, vHTS and Scaffold Hopping, Predictive Science (Biological Activity, ADMET). Structure based Drug Design (SBDD), Lead Optimization, Structural Mining: Protein Ligand work analysis. Study of drug-interactions, Docking.

MODULE 4: 10 Hours
Perl and Bioinformatics: Basics of PERL, Intermediate and Advanced, Biological databases, Sequence analysis and alignment, Evolutionary analysis. Metabolomics.
Working with Discovery Studio (Molecular Modeling): 2D and 3D visualization, 2D and 3D molecular descriptors, Quantum mechanics / molecular, mechanics. SAR analysis, 2D and 3D QSAR. Bayesian statistics, neural networks, recursive partitioning, GFA, etc. Library analysis and Library design. Predictive ADME and toxicology (TOPKAT®), Conformation generation and Analysis. Structure-based and structure-guided design, docking, scoring. Virtual screening and compound ranking/scoring.

MODULE 5: 10 Hours
Receptor-ligand interactions analysis, Fragment-based design, de novo design (LUDI), Pharmacophore generation (Catalyst), Scaffold hopping, 3D database screening, Simulations, molecular mechanics/dynamics (CHARMm). Explicit/implicit solvation models, Transmembrane protein modeling, Homology modeling, Antibody modeling, Electrostatics calculations, protein ionization and pK prediction, Protein modeling (MODELER®) and analysis, protein engineering. Protein-protein docking and refi nement, Sequence analysis, sequence alignment, phylogenetic analysis, • X-Ray (CNX), structure refi nement and analysis.

COURSE OUTCOMES:
After completion of the course, students are able to explain the overview of bioinformatics and biological databases, use the
knowledge of docking to know the 3D structure of receptor; its scope and limitation.

TEXT BOOKS:
1. David W. Mount. “Sequence and Genome Analysis”, Bioinformatics CSHL Press, 2nd Ed., 2004.
2. Baxevanis and F. B. F. Ouellette. “Bioinformatics: a practical, guide to the analysis of genes and proteins”, JohnWiley, 2nd Ed., 2001.
3. Jonathan Pevsner. “Bioinformatics and Functional Genomics”, Wiley-Liss, 1st Ed., 2003.

REFERENCE BOOKS:
4. Setubal Joao and meidanis Joao, Introduction to Computational Molecular Biology, Publisher: PWS Publishing; 1st edition, 1997.
5. R. Durbin, S. Eddy, A. Krogh and G. Mitchison, Biological Sequence Analysis, Cambridge. Cambridge University Press, 1998.
6. Paul M. Selzer, Richard J. Marhöfer, Andreas Rohwer, Applied bioinformatics: an introduction, Berlin Springer 2008.

ENVIRONMENTAL BIOTECHNOLOGY
Subject Code : 14BBT251

IA Marks : 50
No. of Lecture Hrs./ Week : 04 Exam Hrs : 03
Total No. of Lecture Hrs. : 50 Exam Marks : 100

COURSE OBJECTIVES:
To explain and evaluate sustainable and cleaner environment,
To harness renewable enrgy and energy conversions
To asses environmental impact and audit.

MODULE 1: 10 Hours
Concept and scope of environmental biotechnology. Elements of life and biodistribution of elements. Microbiology of air, water & soil, interactions among soil microorganisms. Role of environmental biotechnology in sustainable development.
Waste Water Biotechnology: Nature and components of Waste water, Eutrophication – causes: detergents, effects and control. Waste water treatment – primary waste water treatment: unit operations, secondary waste water treatment: design and modeling of activated sludge process – aerobic and anaerobic, and tertiary waste water treatment – reverse osmosis and ultra filtration, microbial removal of phosphorous and nitrogen. Wastewater composition and treatment strategies in food processing & pharmaceutical industries – sugar factories, vegetable oil industries, potato processing industries, dairy industries, beverages industries distilleries & pharmaceuticals.

MODULE 2: 10 Hours
Solid Waste Management: General composition of urban solid wastes, treatment: aerobic treatment, anaerobic treatment, biogass generation, solid waste management through biotechnological processes involving – hazardous wastes, Biomedical wastes, Dairy wastes, Pulp industry wastes, Textile industry wastes, leather industry wastes and pharmaceutical industry wastes, petroleum wastes treatment. Integrated waste management, super critical water oxidation of wastes.

MODULE 3: 10 Hours
Xenobiotics: Characteristics of Xenobiotics, dose response relationship, effects of xenobiotics, Relationship of Bioaccumulation with Chemical Structures, Eco-physiology of Bioaccumulation – Process of toxicants uptake, Factors affecting bioaccumulation, measurement of bioaccumulation. Degradation of Xenobiotics in the Environment. Biodegradable alternatives for xenobiotics. Biomonitors of environment, Bioremediation using microbes, Phytoremediation.
Bioleaching & Biomining: Biometallurgy, Microbes in Bioleaching and Metal Recovery: Microbial recovery of phosphate, microbial extraction of petroleum and microbial production of fuels.

MODULE 4: 10 Hours
Energy and Environment: Renewable and non-renewable resources. Conventional fuels and their environmental impacts. Modern fuels and their environmental impacts. Biotechnological inputs in producing good quality natural fibers. Plant sources like Jetropha, Pongamia etc. Waste as an energy core, energy recovery systems for urban waste, technology evaluation, concept of gasification of wastes with molten salt to produce low-BTU gas; pipeline gas from solid wastes by syngas recycling process; conversion of feedlot wastes into pipeline gas; fuels and chemicals from crops, production of oil from wood waste, fuels from wood waste, methanol production from organic wastes. Gasohol, Dye-pigments, sensitized solar cells.

MODULE 5: 10 Hours
Green Technology and Environmental Protection: Principles of green technology, enzyme based detergents, alternative pesticides, alternative fuels, bio polymers, techniques and directions in practicing green technology, super critical liquid carbon dioxide solvent, polylactic acid polymers, use of hydrogen peroxide as a benign bleaching agent in paper industry, enzymatic production of cotton textiles, biodegradable builders in detergents, replacement of wood preservatives and synthesis of specialty compounds.

COURSE OUTCOMES:
After completion of the course, students are able to demonstrate and understand the principle of nonconventional energy, better management practices for better environment.

TEXT BOOKS:
1. Pradipta Kumar Mohapatra, Textbook of Environmental Biotechnology, I K International, 2007
2. Buckingham and Evans, Hazardous Waste Management, LaGrega, 2nd Edition, McGraw Hill International Edition, 2001.
3. Noel De Nevers Air Pollution Control Engineering, 2nd Edition, McGraw Hill International Edition, 2000

REFERENCE BOOKS:
4. Metcalf Eddy, Wastewater Engineering Treatment and Reuse, 4th Edition, Tata McGraw Hill, 2003
5. N K Uberoi, Environmental Management, 2nd Edition, Excel Books publication, 2007
6. Canter, Environmental Impact Assessment, 2nd Edition, McGraw Hill International Edition,1996
7. George Tchobanoglous, Hilary Theisen and Rolf Eliassen. Solid Wastes, McGraw Hill Kogakusha,Ltd.

FOOD BIOTECHNOLOGY
Subject Code : 14BBT252

IA Marks : 50
No. of Lecture Hrs./ Week : 04 Exam Hrs : 03
Total No. of Lecture Hrs. : 50 Exam Marks : 100

COURSE OBJECTIVES:
Understand the components of food and principles of food spoilage and techniques for food preservation. Know the application of biotechnology for food preservation and food production with improved nutritional benefits

MODULE 1: 10 Hours
Basic Constitutes of Food: Basic constituents of food, colloidal systems in food, molecular stability of colloidal systems, types of food starches, soluble fibers: pectin’s, mucilage & gums, protein rich foods, oils in foods.
Food Microbiology: Microbial growth pattern, types of microorganisms associated with food: mold, yeast and bacteria. Contaminants of food stuff, milk and meat during handling and processing. Mechanism of food spoilage. Biochemical changes caused by microorganism. Determination of various types of food products. Food borne intoxicants and mycotoxins

MODULE 2: 10 Hours
Food Preservation Technology: Food preservation by high and ultra high temperatures- canning, drying. Food dehydration: .
Equipments for food dehydration: fixed tray dehydration, cabinet drying, tunnel drying. Freeze dehydration, controlled atmosphere, storage, Food preservation by irradiation treatment. Preservation by freezing and refrigeration. Frozen foods. Thermal properties of frozen foods. Food freezing equipments: Air blast freezers, plate freezers and immersion freezers. Preservation by Chemicals and Bacteriocins

MODULE 3: 10 Hours
Food Production Technology: Importance of food industry, specific objectives of food processing, impact of food processing on food constituents. Production of single cell protein, Tailoring of milk proteins and milk fats, Production of fermented food products: yoghurt, pro-biotic cheese, nutritional value, labeling of constituents: Soya foods, organic foods, dietary foods, nutritional food supplements, Use of plant cell culture for the production of food additives (Vanillin, Capsaicin), microbial transformations, regulatory and social aspects of BT. Food packaging, edible films, Marketing of food and promotional strategies.

MODULE 4: 10 Hours
Biotechnology for Improved Processing: Role of biotechnology in food industry, maintenance of nutritional quality, Enzymes in bakery and cereal products, utilization of hydrolases and lipases enzymes. Applications of immobilized enzymes in food industry, enzymes for enhanced flavor and aroma compounds, enzymes in fat and oil industries. Genetically modified plants for high nutritional food.

MODULE 5: 10 Hours
Food Quality Assurance and Control: Importance and functions of quality assurance and control: Methods of quality, concept of rheology, assessment of food materials- fruits, vegetables, cereals, dairy products, meat and processed food products. Microbiological safety of food products, chemical safety of food products, contaminants by heavy metal, fungal toxins and pesticide residue. Food regulations, grades and standards, USFDA/ ISO 9000 Series. Food adulterations and safety, sensors and instrumental analysis in quality control food laws and standards.

COURSE OUTCOMES:
After completion of the course, students are able to enlighten with comprehensive knowledge of biotechnological applications to food industry.

TEXT BOOKS:
1. James M, Jay. Food Biotechnology CBS Publishers 2nd edition, 2005.
2. Kalidas shetty Food Biotechnology, CRC Press. 1st ed. 2005
3. T.Britze, R.K Robinson., Advanced Dairy Science and Technology. Wiley- Blackwell publisher. 1st edition. 2008

REFERENCE BOOKS:
4. H. Elmer, L James, Marath and Steele. Applied dairy microbiology, CRC press 2nd edition 2005.
5. R. Paul Singh., “Introduction to Food Engineering”, Academic Press, 3rd Ed., 2004.
6. P. Fellows, “Food Processing Technology: Principles and practice”. Woodhead Publishing Ltd., Cambrideg, 2nd Ed., 2005.

BIOPROCESS TECHNOLOGY
Subject Code : 14BBT21

IA Marks : 50
No. of Lecture Hrs./ Week : 04 Exam Hrs : 03
Total No. of Lecture Hrs. : 50 Exam Marks : 100

COURSE OBJECTIVES:
To understand the basic concept of bioprocessing along with its kinetics and interaction
Conceptualise the principle and mode of fermenter/bioreactors in accordance to its application
To comprehend the product formulation along with energy dynamics and its utility

MODULE 1: 10 Hours
Bioprocess development: An interdisciplinary challenge, Biotechnology & Bioprocess Engineering, steps in bioprocess development, Media ingredients, medium formulation, media sterilization, medium optimization, Ingredients for mammalian cell culture and plant cell culture. Microbial kinetics: Growth yield, kinetic models, monod kinetics, transient growth kinetics, filamentous growth kinetics, substrate degradation and product formation kinetics, factors effecting kinetics.

MODULE 2: 10 Hours
Fermentation process: Parts of fermenter: Body construction, Baffles, agitators, Sparger, valves, ports. Bioreactor configurations: Bubble column, airlift reactor, packed bed, fluidized bed, trickle bed, Solid state fermenter, Animal and plant cell bioreactors. Scale up of bioreactors. Numericals on scale-up.
Concept of bioprocess control, Elements of feedback controller, types of controller action, advanced control strategies, controller tuning, online and offline measurements (P,T, pH, agitator speed, DO, off gas analysis).

MODULE 3: 10 Hours
Heat and Mass Transfer: Heat transfer in bioreactor, effect of heat transfer on microbial growth, role of diffusion, types of mass transfer (S-L, L-L, G-L), Oxygen requirements and uptake by cells, measuring DO, determination of KLa, factors affecting KLa, oxygen in large vessel. Numericals. Fermenter fluid rheology: Viscosity, broth rheology, viscosity measurement, factors affecting viscosity, mechanism of mixing, flow patterns in fermenter, mixing time, power requirement, effect of rheology on mixing, role of
shear. Numericals.

MODULE 4: 10 Hours
Downstream processing in Bioprocess technology. Process design criteria for various classes of bioproducts (high volume, low value products and low volume, high value products), General account of downstream processing steps: removal of insoluble’s, cell disruption, isolation,

MODULE 5: 10 Hours
Heterogeneous system: Interaction b/w mass transfer and reaction, steady state shell balance, external and internal mass transfer effects. Numericals .
Product purification and product formulation, Quality analysis: Analysis of product purity: Chromatography, electrophoresis and spectroscopy.

COURSE OUTCOMES:
After completion of the course, students are able to demonstrate and apply the principles techniques and law of bioprocess in understanding and designing of fermenters along with product formulation with strong understanding of the concepts behind.

TEXT BOOKS:
1. Stanbury & Whittaker, Principles of Fermentation Technology. Pergamon Press, 2000.
2. Pauline M. Doran. Bioprocess Engineering Principles. Academic Press, 2003
3. E. I. Mansi & Bryce. Fermentation Microbiology & Biotechnology. Taylor & Francis, 2004.

REFERENCE BOOKS:
4. Bailey & Ollis. Biochemical Engineering Fundamentals, McGraw Hill, 1986.
5. Shuler and Kargi. Bioprocess Engineering. Prentice Hall, 1992.
6. Hans-Peter Schmauder. Methods in Biotechnology. Taylor & Francis, 2004

BIOPROCESS AND BIOPHARMACEUTICAL LAB
Subject Code : 14BBT26

IA Marks : 25
No. of Lecture Hrs./ Week 03 Exam Hrs : 03
Exam Marks : 50

COURSE OBJECTIVES:
To provide hands on training with procedures for real time problems in bioprocess and biopharmaceutical Technologies.

Lab Experiments
1. Determination of thermal death kinetics of microorganism
2. Batch and Fed-batch growth kinetics
3. Media optimization.
4. Determination of KLa.
5. Product separation & concentration methods
6. Chromatographic techniques
7. Isolation, propagation and preservation techniques for cells.
8. Expression studies using prokaryotic microbial system (e.g. E.coli or Bacillus)
9. Expression studies using eukaryotic microbial system (e.g. Saccharomyces / Pichia)
10. Small scale liquid fermentation for therapeutic production
11. Solid state fermentation techniques.
12. Purification and characterization of proteins.

COURSE OUTCOMES:
At the end of the sessions the student will be able to perform laboratory experiments related to Bioprocess Engineering and gain experience in laboratory methods for Biopharmaceuticals. Also will be able to contribute to these domains.

TEXT / REFERENCE BOOKS:
1. Methods in Biotechnology Editor: Hans Peter Schmauder. Published by Taylor & Francis. 2004.
2. Manual of Industrial Microbiology and Biotechnology. Editors: Arnold Demain and Julian Davies. 2004.
3. Stanbury & Whittaker, Principles of Fermentation Technology. Pergamon Press, 2000.
4. Pauline M. Doran. Bioprocess Engineering Principles. Academic Press, 2003

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